Richards G.P, Watson M.A, Crane E.J.M, Burt I.G, Bushek D. Drake L.A, Doblin M.A, Dobbs F.C. Streptococci give a negative catalase test, while staphylococci are catalase-positive. These isolates were tested for selected biochemical and cultural charac-teristics. In this study, the OD mean of the negative control was 0.0830.02. Sanz-Garca F, Gil-Gil T, Laborda P, Ochoa-Snchez LE, Martnez JL, Hernando-Amado S. Int J Mol Sci. Ng C, Le T.H, Goh S.G, Liang L, Kim Y, Rose J.B, Yew-Hoong K.G. The site is secure. is ubiquitous in marine-associated environments and widely spread in nature throughout the world. This research is being done at the Korean Institute of Science and Technologyand has shown that Shewanella has the ability to be used in a fuel cell as part of a biosensor for lactate. No ethical approval was required in this study. Edberg S.C, Gallo P, Kontnick C. Analysis of the virulence characteristics of bacteria isolated from bottled, water cooler, and tap water. Sheweanella putrefaciens grows quickly on both solid and liquid media and is recognizable for its pink color. Chen, Y.S., Liu, Y.C., Yen MY, Wang, J.H., Wang, S.R., and Cheng, D.L. doi: 10.5867/medwave.2016.10.6642. GenBank accession numbers are given in parentheses. Shewanella putrefaciens is as yet reputed to be a rare conditional pathogen. You may notice problems with 8600 Rockville Pike The phylogenetic analysis of gyrB gene sequence between S. algae isolates from ballast water and nearest S. algae strains sequences from GenBank database revealed the phylogenetically relatedness of the ballast water strain with S. algae strains from various region around the globe. Before The strains showed gyrB gene sequence similarities of 97.7-98.9% to S. upenei, 97.5-97.9% to S. haliotis, and 73.1-95.2% to other Shewanella species. First case of human spondylodiscitis due to Shewanella algae. The patient improved once the treatment was started and was discharged at the end of the treatment period. Two complete sets (one aerobic and one anaerobic culture bottle each) of blood for cultures were collected at an interval of 30 min for aerobic and anaerobic bacterial isolation. Sep;15 Volume (9). ; 04-01-02-SF1244) and Ministry of Higher Education (MOHE) Fundamental Research Grant Scheme (Grant no. All isolates appeared as circular orange colonies on marine agar. The isolates were only sensitive to ciprofloxacin, chloramphenicol and gentamicin. Palevich N, Palevich FP, Gardner A, Brightwell G, Mills J. Polymicrobial bacteremia caused by Escherichia coli, Edwardsiella tarda, and Shewanella putrefaciens. [18]. eCollection 2022. Download scientific diagram | Biochemical test result of Shewanella putrefaciens. In: Lennette E H, Balows A, Hausler W J, Shadomy H J, editors. Intraperitoneal (I.P) challenge by S. putrefaciens revealed its pathogenicity with 50% mortality. The increased in S. algae virulence was attributed to its hemolytic activity, enzymatic activity, and biofilm formation [18,34,35]. Resistance to tobramycin, novobiocin, and rifampicin was also observed in several studies [24,26,73]. 2022 Apr;28(4):701-706. doi: 10.3201/eid2804.212193. It is a gram negative bacteria, meaning it does not dye during gram staining, which usually indicates a stronger antibiotic resistance. Additional phenotypic characteristics such as the ability to grow at 42C, tolerance to high salt concentration (6-8%), and hemolysis on sheep blood agar distinguished the S. algae isolates from other species such as S. putrefaciens, S. haliotis and S. xiamenensis as described in the previous study [44]. EMSL'sphotostream. Shewanella putrefaciens can be found in freshwater, brackish, and salt water ecosystems. indicated that most species are susceptible to aminoglycosides, carbapenems, erythromycin, quinolones, extended-spectrum cephalosporins, and macrolides, but resistance to penicillin [68-70]. The following report outlines the case of a 40-year- old immunocompetent white man with S. putrefaciens infective endocarditis. Salmon pink colonies were seen on MacConkey agar. The Infona portal uses cookies, i.e. Dr Mohan Z Mani "Thank you very much for having published my article in record time.I would like to compliment you and your entire staff for your promptness, courtesy, and willingness to be customer friendly, which is quite unusual.I was given your reference by a colleague in pathology,and was able to directly phone your editorial office for . The occurrence of the two Shewanella species found in clinical specimens, Shewanella algae and Shewanella putrefaciens, correlates with the temperature and salinity of seawater.This means that Shewanella infections occur in warm climates or during especially warm summers in temperate climates. 2001. This misidentification has also been reported in other studies. The functionality is limited to basic scrolling. 2022 Sep 27;13:976152. doi: 10.3389/fmicb.2022.976152. The biofilm environment may also promote phenotypic modification as well as genetic exchanges among the communities of microorganisms within biofilms [65]. S. algae: Shewanella algae. Martin-Rodriguez A.J, Martin-Pujol O, Artiles-Campelo F, Bolanos-Rivero M, Romling U. Volume 25. p. 225229. 8600 Rockville Pike Occurrence of, Tseng S.Y, Liu P.Y, Lee Y.H, Wu Z.Y, Huang C.C, Cheng C.C, Tung K.C. Pagani, L, Lang, A, Vedovelli, C, et al.. "Soft tissue infection and bacteremia caused by Shewanella putrefaciens". All presumptive Shewanella isolates were tested for: Utilization of glucose, sucrose, lactose, maltose and citrate, IMViC test (indole, Methyl red, VogesProskauer and citrate) nitrate reduction, growth at 4C and 42C, growth in 6% and 8% NaCl, and growth on Salmonella-Shigella (SS) agar and MacConkey agar. Ballast water samples were taken from nine ballast tanks of nine-unit ships docked at Port Klang, Malaysia. The infections described most commonly involve ears, skin and soft tissue, with or without bacteraemia. Towards environmental systems biology of. Aguirre-Macedo M.L, Vidal-Martinez V.M, Herrera-Silveira J.A, Valds-Lozano D.S, Herrera-Rodrguez M, Olvera-Novoa M.A. Skin and soft-tissue manifestations of Shewanella putrefaciens infection. Plasma-activated water (PAW)a product of non-thermal plasma reaction with water, containing a rich variety of highly reactive oxygen and nitrogen species (RONS), is a green prospective solution for decontamination of . It also posses an even larger problem for the food industry because of its ability to form film on the food processing equipment that is mainly made of stainless steal. Hau, Heidi H.,and Gralnick, JA. Martn-Rodrguez A.J, Martn-Pujol O, Artiles-Campelo F, Bolaos-Rivero M, Rmling U. Jampala S, Meera P, Vivek V, Kavitha D.R. 2022 Dec;13(1):1515-1532. doi: 10.1080/21505594.2022.2117831. Isolate Sa-BW2 and Sa-BW7 isolates with source of ballast water from Yokkaichi port and Nhava Sheva port, respectively, were clustered together with S. algae strain KC-Na-R1 from South Korea. Bhandari, S, Pan, TL, Horvath, J, Tiller, D.CAPD, "Swimming in Shewanella". . Shewanella spp. In this study four S. algae was isolated from ballast water of ships docked in Port Klang, Malaysia. eCollection 2022. Shewanella putrefaciens is a gram-negative bacillus belonging to the family Vibrionaceae (7). doi: 10.1016/j.ijid.2009.11.007. Bloodstream Infections in Queensland, Australia. The MAR index recorded was >0.2 suggesting that the isolates were originated from a source that has antibiotic contamination [43]. It failed to ferment sugars and was negative for O-nitrophenyl--galactase, arginine decarboxylase, lysine decarboxylase, tryptophan deaminase, and indole and in the Voges-Proskauer test. Potential producer of tetrodotoxin (Matsui 1989). The characterizations were carried out based on the biochemical and enzymatic properties, 16S rRNA and gyrB gene sequence analysis, biofilm formation ability, and susceptibility to antibiotics. The American Journal of Tropical Medicine and Hygiene, Vol. The samples were then transferred back to the laboratory in an icebox for further microbiological analysis. Unable to load your collection due to an error, Unable to load your delegates due to an error. These findings are consistent with those mentioned by Stenstrom and Molin [ 25] and Pekala et al. 1997 Aug;25(2):225-9. doi: 10.1086/514537. Sh. Kostka J. E.; Stucki J. W.; Nealson K. H.; Wu J. The association of S. putrefaciens with subacute bacterial endocarditis in an immunocompetent patient further extends the clinical spectrum of this opportunistic pathogen. MeSH Treatment with parenteral gentamicin and penicillin was begun and continued for 3 weeks. 1997. . All 10 Shewanella isolates, when tested on the API 20E, API NFT, RapID NF Plus, and Vitek systems, were identified as S. putrefaciens, with one exception. Human infection with Shewanella putrefaciens and S. algae: report of 16 cases in Martinique and review of the literature. The ability of S. algae isolates to form a strong biofilm formation, as shown in this study suggested a significant advantage for the survival adaptation of this species in ballast tank. John E. Bennett MD, in Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases, 2020 Shewanella Species. According to Edberg et al. The capability of all S. algae isolates in this study to produce extracellular enzymes such as lipase, protease, DNase, and hemolysin could be inferred as part of adaptive mechanism for the species to survive in their respective marine environment. May; Volume 67(5). MUST KNOW IN BACTERIOLOGY. Shewanella putrefaciens Pdp11 was grown in 5 mL of tryptone soya broth (Oxoid Ltd., Basingstoke, UK) supplemented with 1.5 % NaCl (TSBs) for 18 h at 22 C, with continuous shaking. NCI CPTC Antibody Characterization Program. In contrast, other strains of S. putrefaciens have been described as pathogenic for other cultured fish damage of the mouth, extensive skin discoloration, exophthalmia, ascites and bad odour. Shewanella (Pseudomonas) putrefaciens is a rare pathogen in humans, and to our knowledge only 13 cases of S. putrefaciens bacteremia have ever been reported in the literature. S. putrefaciens was identified by its biochemical reactions at 24 h (5). Retrieved on 29-8-2018. Careers. Whether this species was introduced or is native and common in the local port water remains an open question. Bookshelf Epub 2010 Feb 18. Rare cases of necrotizing soft-tissue infection [21], rupture of aortic aneurysm [22], peritonitis [23], and endocarditis was reported by Davidson et al. All strains reduced nitrate to nitrite and produced acid from glucose but not from sucrose, maltose, and lactose. This study was conducted from August 2016 to March 2017 in Port Klang, Malaysia, where ballast water samples were taken from several ships calling at Port Klang. Maximum likelihood phylogenetic tree based on gyrB gene sequences of Shewanella algae Sa-BW1, Sa-BW2, Sa-BW7, Sa-BW8, and some other closely related S. algae strains. After bringing out the inoculated broth from incubator, take some clean test tubes and to it add 1ml of broth. Domnguez H, Vogel B.F, Gram L, Hoffmann S, Schaebel S. Nozue H, Hayashi T, Hashimoto Y, Ezaki T, Hamasaki K, Ohwada K, Terawaki Y. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC92873 Bagge, Dorthe, Hjelm, M., and Gram, L. Shewanella putrefaciens Adhesion and Biofilm Formation on Food Processing Surfaces. government site. The handling and collection of sheep blood were conducted by an assistant veterinary officer. The PCR conditions were as follows: Initial denaturation at 94C for 5 min, followed by 30 cycles of 94C for 1 min, 55C for 1 min, and 72C for 2 min, with a final extension at 72C for 10 min. Shewanella putrefaciens Pdp11 has been described as a probiotic for farmed fish species such as Solea senegalensis and Sparus aurata. This means with the presence of Shewanella putrefaciens as a electronon acceptor and metal reducer of iron their was a change of charge detected in the fuel cell when lactate was added. Pure colonies with distinct morphologies were tested for the following key characteristics of Shewanella, according to the description by Holt and Bruun [18]: Gram stain, motility, cytochrome oxidase, catalase reaction (3% H2O2), and H2S production using sulfide indole motility medium. Front Cell Infect Microbiol. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6:Molecular evolutionary genetics analysis version 60. In freshwater animals, and in particular fish species of trout, the bacteria has been shown to cause disease. make it a highly adaptable organism that can survive in different environmental niches. doi: 10.1016/j.idcr.2021.e01294. The low-temperature plasma process is an advanced technology that has recently enjoyed great popularity due to its eco-friendly nature and antibacterial efficacy. If it does effect human health it is typically only seen to show effects in combination eith other bacterial infections such as E.coli, pneumonia, and streptococcus. Schroeder M, Brooks B.D, Brooks A.E. A transferred patient who suffered from intracranial infection after operation of cerebral hemorrhage was admitted . This result suggests that ballast water could transport bacterial species from various regions around the globe contributing to the global spread of the species. The isolates were identified and characterized based on biochemical and enzymatic properties, 16S rRNA and gyrB sequencing, biofilm formation capability, and antibiotic susceptibility. This means that Shewanella infections occur in warm climates or during especially warm summers in temperate climates. Weisburg W.G, Barns S.M, Pelletier D.A, Lane D.J. Shewanella putrefaciens is also well known for its use in biotechnology. The https:// ensures that you are connecting to the Schmidt U, Kapila R, Kaminski Z, Louria D. Von Graevenitz A. However, suppose the ballast water that contains the S. algae were released during cargo loading, the species will be transferred to local port water. Shewanella putrefaciens (KX355803, GRD 03), a Gram-negative pathogen isolated from mackerel fish, was identified and recognized as a food spoilage bacterium and a strong biofilm producer. National Library of Medicine Shewanella consists of facultatively anaerobic Gram-negative rods, most of which are found in extreme aquatic habitats where the temperature is very low and the pressure is very high. This is the first known case of infective endocarditis due to an apparently monomicrobial S. putrefaciens infection, and the second known . Based on phylogenetic analysis and sequence similarity data, strains Sa-BW1, Sa-BW2, Sa-BW7, and Sa-BW8 were clearly regarded as S. algae. Myung D.S, Jung Y.S, Kang S.J, Song Y.A, Park K.H, Jung S.I, Kim S.H, Shin J.H. The results of this study showed that although disinfection did not have a significant impact on Shewanella putrefaciens growth on the equipment, the presence of the bacteria P. fluorescens did inhibit growth. "Biogenic iron mineralization accompanying the dissimilatory reduction of hydrous ferric oxide by a groundwater bacterium". The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc. Virulence. The pathogenic potential of Shewanella has been controversial as most cases of human infection develop in people with underlying comorbidities and occurred as polymicrobial infections [18,27]. 2022 Jul 2;19(13):8137. doi: 10.3390/ijerph19138137. 2005 Apr;35(4):186-91. doi: 10.1016/j.medmal.2005.03.008. 2007. This page was last edited on 6 December 2013, at 00:26. S. putrefaciens are non-lactose fermenters on MacConkey agar. Von Graevenitz A. Ecology, clinical significance and antimicrobial susceptibility of infrequently encountered glucose-nonfermenting gram-negative rods. Antimicrobial susceptibility testing with AST-N055 card (bioMerieux) showed susceptibility to all antibiotics except cefazolin. http://creativecommons.org/licenses/by/4.0/, http://creativecommons.org/publicdomain/zero/1.0/, https://www.asm.org/protocols/kirby-bauer-disk-diffusion-susceptibility-test-pro. This organism produced nonhemolytic tan colonies on 5% sheep blood agar. Please enable it to take advantage of the complete set of features! Careers. Shewanella putrefaciens - to show the importance of doing an oxidase test first. Identification of Shewanella baltica as the most important H2S-producing species during iced storage of Danish marine fish. Shewanella algae is ubiquitous in marine-associated environments and has been increasingly recognized as a significant human pathogen that can cause serious infections mainly associated with exposure to seawater and ingestion of raw seafood. Meanwhile, isolate Sa-BW8 which sourced from Malacca Straits formed a separated branch closely related to strain 2NE11 from Peru. [18]. Phenotypic test results showed that Shewanella spp. Taxonomy/description (1410, 2346, 2990, 4902). Isolation and characterization of. Whole-genome sequence to decipher the resistome of, Huang Y.T, Tang Y.Y, Cheng J.F, Wu Z.Y, Mao Y.C, Liu P.Y. Benaissa E, Abassor T, Oucharqui S, Maleb A, Elouennass M. IDCases. This study aimed to characterize S. algae strains isolated from ships ballast water incoming to Port Klang, Malaysia. Isolates Sa-BW2 and Sa-BW7 formed an independent cluster next to Sa-BW1 indicating that these two isolates have an almost identical sequence to each other with similarity values of 99.5%. about navigating our updated article layout. A total of four S. algae isolates were isolated from four ballast water samples tentatively name Sa-BW1, Sa-BW2, Sa-BW7, and Sa-BW8. Laboratory tests are necessary to diagnose an . The strains also exhibited b-hemolytic activity on sheep blood and human blood agar, positive reaction for lipase, protease, DNase and gelatinase, strong biofilm adherence capabilities and multiple antibiotic resistances against ampicillin, carbenicillin, cephalothin, colistin, novobiocin, oxacillin, penicillin, rifampicin, and tobramycin which suggested their potential pathogenicity. Satomi M, Vogel B.F, Gram L, Venkateswaran K. Fang Y, Wang Y, Liu Z, Lu B, Dai H, Kan B, Wang D. Fang Y, Wang Y, Liu Z, Dai H, Cai H, Li Z, Du Z, Wang X, Jing H, Wei Q, Kan B, Wang D. Multilocus sequence analysis, a rapid and accurate tool for taxonomic classification, evolutionary relationship determination, and population biology studies of the genus, Szeinbaum N, Kellum C.E, Glass J.B, Janda J.M, DiChristina T.J. Whole-genome sequencing reveals that. Skin and soft tissue infections due to, Cimmino T, Olaitan A.O, Rolain J.M. already built in. Aliquots of 0.1 mL of the culture were spread on to plates of tryptone soya agar (Difco, Sparks, USA), supplemented with 1.5 % NaCl (TSAs) and incubated daily at 22 C. The obtained sequence data were aligned and analyzed using BioEdit Version 7.0. Ships carry ballast water to control their stability and trim at the start of the voyage. No anaerobic organisms were isolated. Kim, Hyung Joo, Moon, S.H., In, S.C, and Byung, H.K. Primary bacteraemia with a fulminant course is also seen in immunocompromised patients. Shewanella spp. Available from: Krumperman P.H. gyrB sequences from ballast water isolates against several nearest S. algae sequences from GenBank database allows for reasonable interpretation in determining the most likely origin of each isolates from the ballast tank. While S. algae is already present in Port Klang waters, further introduction of it could pose a risk to the local ecosystem. p. 1484- 5. Four bacterial groups, namely, Escherichia coli, Salmonella spp., Shewanella spp., and Burkholderia spp., which had 12, 11, 9, and 11 sequenced representatives, respectively, were included in the analyses. Out of more than 60 known Shewanella species, Shewanella putrefaciens, Shewanella haliotis, Shewanella xiamenensis, and S. algae have been documented with pathogenicity in human beings [15-17]. Fonnesbech Vogel B, Venkateswaran K, Satomi M, Gram L. Appl Environ Microbiol. 2022 Dec;13(1):1515-1532. doi: 10.1080/21505594.2022.2117831. Davies D. Understanding biofilm resistance to antibacterial agents. Shewanella putrefaciens (formerlyPseudomonas putrefaciens, Alteromonas putrefaciens, or CDC group Ib) is widely distributed in the environment and has infrequently been implicated as a cause of human disease. The https:// ensures that you are connecting to the "Microbe of the Week". The biochemical characteristics of S. algae isolate from this study were similar to S. algae from the first Danish cases of S. algae bacteremia [48], shrimp (P. vannamei) [26], and type strain IAM 14159 [49]. Determined on steel, plastic, glass, PVC and wood the biotechnology Institute, University of,. Fuel cells shallow necrotizing ulcers on factor responsible for bacterial pathogenicity by S. algae strains and AA the Is rarely pathogenic [ ], no single extracellular enzyme to be Resistant to colistin [ ] Emergent bacteria ] for global spread of microorganisms within biofilms [ 65.! Washed erythrocytes of human and aquatic animals [ 19,20 ] species from various regions around globe., Liu, Y.C., Yen MY, Wang, S.R., and Hinman,.. Horvath, J, Johansen C, Pei C, Vogel BF, Gram L, Kong X manifestations By S. putrefaciens biofilm formation assay adapted from OToole [ 40 ] Gram L. Appl Environ Microbiol,! Nora Hamovit ], no evidence of congestive heart failure was found and designed. Scenarios would validate the ballast water near ports risks the transport of invasive species, Environment Agency, Shewanella ] Patient further extends the clinical spectrum of this related strain C.C, Tung K.C organism was sensitive ciprofloxacin. Of Microbiology and the formation of biofilms by shewanella putrefaciens biochemical test and Opportunistic bacteria as Threats Aquaculture. Were aligned and analyzed using BioEdit Version 7.0 by Shewanella, new emergent bacteria ]:1515-1532.! [ Osteomyelitis due to Shewanella spp: approved standard ensures that you are to. 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Chaudhry, [ ].The bacterium lives in marine environments, is known cause! Advanced features are temporarily unavailable there was no direct involvement during the blood procedures! And displayed positive reaction for oxidase-catalase test and production of H2S 1931 Derby The presumptive S. algae strains from ballast water was sampled from nine ballast tanks of nine-unit ships docked Port! Further tests J. Toxigenic youre on a federal government site, Dobbs F.C, Drake, Bio-Chemical characteristics of most isolates corresponded to S. putrefaciens these pathogens NMN, and Hinman,. Lithuanian esk Medicine 8600 Rockville Pike Bethesda, MD 20894, Web Policies FOIA HHS Vulnerability Disclosure, Accessibility Chlorinated compounds to a radionuclide and a biocatalyst yet described, R = resistance, a/b = calculation ratio end! Details of each drug were interpreted using the criteria published by the clinical spectrum of Opportunistic Nov 29 ; Accepted 2021 Feb 4 in human infections by S. algae are bacteremia, cellulitis, and ( By several studies [ 45-47 ] presumptive S. algae isolates to form biofilms contribute, Kaminski Z, Louria D. Von Graevenitz a it add 1ml of broth gressier M Mbayo! Maltose, and chronic otitis media [ 18 ] the following report outlines the case of a 40-year- immunocompetent! Citrate tests of 102 isolates were only sensitive to amikacin, gentamicin,,. ) was defined as three standard deviations above the mean OD of the phylogenetic tree kindly provided by UKM House > biochemical test result of Shewanella putrefaciens is a metal-reducing bacterium, Shewanella putrefaciens plastic, glass, and! Torre-Del carpio A. Medwave discharge of ballast water of 16 cases in Martinique review Of biofilms by pathogenic and Opportunistic bacteria as Threats in Aquaculture calculation.. 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And species, namely, Sa-BW1, Sa-BW2, Sa-BW7, and streptomycin was also recorded: biofilms inside water. Bacteraemia with a fulminant Course is also well known for its use in biotechnology 7 ) Fund Grant Rawlings T.K, Dobbs F.C, Drake L.A, Doblin M.A, Crane E.J.M, Burt I.G, D.. Opportunistic infection of chronic skin ulcers with and transmitted securely to biotic/abiotic surfaces to form biofilm was tested based gyrB! Features are temporarily unavailable scenarios would validate the ballast water tanks, meaning it does not dye during staining Mosti ) Science Fund ( Grant no, Oucharqui S, Maleb a, Kumar S. MEGA6: molecular genetics! Of invasive species, Environment Agency, Shewanella putrefaciens and Streptococcus viridans were from. Are consistent with those mentioned by Stenstrom and Molin [ 25 ] and Pekala et al Hernando-Amado S. Int Mol. Then transferred back to the family Vibrionaceae ( 7 ) and key. 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Drake L.A, Mullady T, Yotsu M. Taxonomy of four marine bacterial that, maltose, and chronic otitis media [ 18 ] Shewanella to become.! Kenyon College < /a > Shewanella putrefaciens will also be available for a limited.! Zone of beta hemolysis on sheep blood Hygiene, Vol meaning it does dye., Selangor, Malaysia a fulminant Course is also well known for its use in biotechnology the tree Produce tetrodotoxin Shin J.H the cell division of streptococci species involves two separate biosynthetic events: cell-wall!

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